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pH Control in Bioreactors
![header_ph_control_2220x740](/media/MAIN/01-Lab-Academy/Bioprocessing/Header-and-icons/Header_ph-control_2220x740_1080.jpg)
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Principle of pH control in bioprocesses
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What different types of pH sensors are available?
Electrochemical pH sensors
- Advantages: The glass membrane material can be adapted to various bioreactor conditions, including extremes in pH or temperature.
- Disadvantages: Glass pH sensors require careful management and can lose accuracy over time, with a need for frequent calibration.
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Optical pH sensors
- Advantages:
- Optical pH sensors can be used in a non-invasive manner that reduces the contamination risk. This is particularly interesting for cell culture applications.
- Optical pH sensors are inexpensive and disposable.
- Disadvantages:
- The measurement range of optical pH sensors is narrower than the range of electrochemical pH sensors. Eppendorf recommends a measurement range of pH 6.5 to 7.5. This is usually sufficient for cell culture applications but may be too narrow for certain microbial applications.
- Certain chemicals including organic solvents can damage optical pH sensors. Additionally, these sensors can measure a relatively limited pH range.
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What are digital pH sensors ?
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pH control strategies
What acid and base should I use for pH control in microbial and cell cultures?
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What is a pH deadband?
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What is the difference between one-sided and two-sided pH control?
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How do you calibrate a pH sensor?
Electrochemical pH sensor calibration
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Optical pH sensor calibration
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How frequently should you calibrate pH sensors?
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How strong is the influence of temperature for your pH calibration?
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Your pH sensor take ages to calibrate, what can you do?
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Bioprocess control systems for streamlined pH control in bioreactors
Eppendorf has a range of bioprocess control systems suitable for both microbial and mammalian cell cultures. They offer high flexibility in pH control. Most systems are compatible with standard electrochemical as well as optical pH sensors and can be used with digital as well as analog sensors. Each bioprocess controller also has the ability to control a range of additional parameters such as temperature and dissolved oxygen , enabling streamlined bioprocess control tailored towards your bioprocessing needs.
![844887_730px_x_411px](/media/MAIN/01-Lab-Academy/Bioprocessing/Photos/844887_730px-x-411px_700.jpg)